Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae), is a recently introduced non-native invasive species in the United States that has the potential to destroy several tree species in urban and forest habitats. The ability to rear A. glabripennis in quarantine is critical to rapid progress on techniques for the exclusion, detection, and eradication of this pest. Survival and development were compared for larvae from two populations (Chicago, IL, and Queens, NY) on six diets containing varying levels of Fe (69–237 mg/liter) and for three populations (Illinois and New York plus Hohhot, Inner Mongolia, China) under four larval chill treatments (6, 9, 12, or 16 wk of development before chill). Larval survival and percentage pupation significantly decreased and developmental time slightly increased with increasing Fe levels in the diet. Larval survival and percentage of pupation were highest, adults weighed the most, and developmental time was shortest when larvae were reared on a pourable modification of the Enaphalodes rufulus (Haldeman) (ER) diet. Individuals from the China and Illinois populations were heavier than those from New York. On average, larvae from the Illinois population were ready to pupate sooner than those from New York or China. Some larvae that had not reached their critical weight for pupation before the chill period required a second chill period before initiating pupation. Overall survival increased as the developmental time period before chill increased. Further evaluation of the effects of temperature on development is needed to better understand the triggers for pupation and to predict the timing of various stages.